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 High Resolution Massively Parallel Holography to Peer Inside The Cell

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Date posted: 22/09/2008

A collaboration of American and European scientists is working on an X-ray holographic technique, which promises the ability to image individual molecules within a living cell.

Using high-energy, extremely short-pulse ? less than 100 femtoseconds, or one quadrillionth of a second ? X-ray beams to examine nanoscale objects is not a new concept. The difficulty lies with the algorithms to convert the resulting patterns into usable images.

One method to increase the signal and resolution of the image is to include a second item with known features during the laser imaging. Known as a ?reference object,? it gives the researchers additional information with which to process the imaging data.

What is new is to use a very special reference object called a ?uniformly redundant array? (URA). In this case, a combination of complex formulas known as a ?Fourier Transform? and a ?Hadamard Transform? are utilized to convert the data into an image that represents the object being examined. Hadamard transforms are commonly used in signal processing and data algorithms, including those used in photo and video compression.

According to Hau-Riege, ?The resolution we achieved is among the best ever reported for holography of a micrometer-sized object, and we believe that it will improve in the future with the development of nano-arrays for Fourier Transform Holography at LCLS.?

See the full Story via external site: www.medgadget.com



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